Oligo LC-MS (Xevo G2)

Image of a Xevo G2 QTof used for oligonucleotide analysis

Location:
Basement MS (00.097)

Training required:
Yes, contact Elisabete Pires

Training prerequisites:
None

Instrument description:

The Waters Xevo G2 QTof delivers exceptional levels of sensitivity, selectivity, in-spectrum dynamic range, speed of analysis, quantitative accuracy and exact mass performance. The Xevo G2‐oligo spectrometer system is coupled to an Acquity UHPLC system, providing ultra‐high sensitivity LC‐MS for the analysis of oligonucleotides. Analyses are completed by LC/MS using an ion-pairing reversed phase method where a mixture of HFIP and TEA is used to elicit retention of the polar polyanionic molecule. A RP‐HPLC purification step of the oligonucleotide sample needs to be performed before using the system. Oligonucleotide analysis requires deconvolution software for the identification of oligonucleotides.

Typical applications:

Oligonucleotide analysis

Agilent GC-MSD User Information

Bruker MALDI Sample Preparation

Combining Trypsin/Chymotrypsin Enzymatic Digestion

In-Gel Trypsin Enzymatic Digestion

In-solution Chymotrypsin Enzymatic Digestion

In-solution protein digestion

MALDI Oligonucleotide Sample Preparation

MALDI User Information

MALDI-MS (Autoflex)

MassLynx

Ms Elisabete Pires

Research Associate in Biological Mass Spectrometry

Native MS Sample Preparation