MALDI-MS (Autoflex)

Location:
Basement MS (00.097)

Training required:
Yes, contact Elisabete Pires

Training prerequisites:
None

Instrument description:

Innovative MALDI-TOF and TOF/TOF technology designed and optimized to be easy-to-use; robust systems for both traditional MALDI applications (reliable and detailed protein/peptide characterization, and polymer analysis) as well as cutting edge work (high resolution MALDI Tissue Imaging, glycan analysis. Autoflex MALDI-TOF/TOF instrument works in linear only mode, high resolution reflectron mode and the latest TOF/TOF technology (LIFT™) which enables the use of various MS/MS techniques (e.g. LID, high energy CID, ISD) for fast and sensitive MS/MS experiments.

Typical applications:

Small molecule analysis, Intact mass determination of proteins (up to 150kDa), Peptide Mass Fingerprint (PMF), In Source Decay (ISD) analysis, Oligonucleotide analysis, Polymer characterization

Agilent GC-MSD User Information

BioTools

Bruker MALDI Sample Preparation

Combining Trypsin/Chymotrypsin Enzymatic Digestion

flexAnalysis

In-Gel Trypsin Enzymatic Digestion

In-solution Chymotrypsin Enzymatic Digestion

In-solution protein digestion

MALDI Oligonucleotide Sample Preparation

MALDI User Information